Unikani njira yogwirira ntchito ndi mfundo zaukadaulo za sitanelo peptide synthesizer

1. Ntchito ndondomeko ya6-channel peptide synthesizer:

1. Konzani zopangira: sankhani utomoni woyenera wa amino acid, magulu oteteza ndi ma condensation reagents. Onetsetsani kuti ma reagents ndi zosungunulira zauma kuti mupewe hydrolysis reaction.

2. Katundu utomoni: Katundu amino asidi utomoni mu zimene ndime ya synthesizer. Utoto ukhoza kugawidwa mofanana mumayendedwe asanu ndi limodzi kuti muwonetsetse kuti kaphatikizidwe kabwino ndi mtundu wa unyolo uliwonse wa peptide.

3. Kuphatikizika kwa amino acid: Sakanizani ma amino acid omwe mukufuna ndi ma condensation reagents oyenerera ndikuwonjezera pazambiri zomwe zachitika. Kulumikizana komwe kumachitika nthawi zambiri kumatenga nthawi kuti zitsimikizire kuti ma amino acid amangika kwathunthu ku utomoni.

4. Kuchotsa Magulu Oteteza: Pambuyo pophatikizana ma amino acid onse, magulu oteteza ayenera kuchotsedwa kuti awonetsere magulu a amino pokonzekera kuzungulira kotsatira.

5. Kutsukidwa ndi kutsekedwa: Pambuyo pa kutetezedwa, utomoni uyenera kutsukidwa bwino ndipo magulu otsalira otsalira ayenera kuchotsedwa kuti asasokoneze zotsatira zotsatila.

6. Kuzungulira motsatizana: Bwerezani masitepe omwe ali pamwambawa mpaka peptide yomwe mukufuna itapangidwa. Kuzungulira kulikonse kumafunika kuwonetsetsa kulumikizidwa kwathunthu kwa ma amino acid ndikuchotsa kwathunthu magulu oteteza.

II.Mfundo zaukadaulo:

1. Kusankha chonyamulira chagawo lolimba: Kusankha chonyamulira chagawo cholimba (mwachitsanzo, utomoni) ndikofunikira pakuphatikiza kwa peptide. Mtundu ndi chikhalidwe cha utomoni zidzakhudza liwiro ndi mphamvu ya kaphatikizidwe.

2. Condensation reaction: Condensation reaction ndi gawo lofunikira pakuphatikizika kwa peptide, ndipo ma reagents ogwira mtima amayenera kusankhidwa kuti atsimikizire kuti kulumikizana pakati pa ma amino acid kumakhala kokwanira komanso kosinthika.

3. Njira zotetezera: Popanga peptide, maunyolo am'mbali a amino acid nthawi zambiri amafunika kutetezedwa kuti asachite mosayenera panthawi ya condensation. Kusankha gulu loteteza loyenera ndikuwongolera mikhalidwe yachitetezo chake ndiye chinsinsi cha kupambana kwa kaphatikizidwe.

4. Kutaya zinyalala: Zinyalala ndi ma reagents osakhudzidwa omwe amapangidwa panthawi ya kaphatikizidwe amayenera kutayidwa moyenera kuti achepetse kuipitsidwa kwa chilengedwe ndikuwonetsetsa chitetezo cha labotale.

5. Kuwongolera Ubwino: Pakapangidwe kake, kuyezetsa kokhazikika kwaubwino kumafunika kuonetsetsa kuti gawo lililonse la zomwe zimachitika likuchitika monga momwe adakonzera komanso kuti peptide yopangidwa imakwaniritsa zomwe zidakonzedweratu komanso zofunikira zachiyero.

Zochita za6-channel peptide synthesizerimafunika kuwongolera bwino kwamankhwala komanso kasamalidwe kokhazikika. Kumvetsetsa bwino kagwiritsidwe ntchito ka synthesizer ndi mfundo zaukadaulo ndikofunikira kuti pakhale luso komanso mtundu wa kaphatikizidwe ka peptide.