Gisesengura imikorere yimikorere ningingo ya tekinike ya peptide itandatu ya peptide

1. Igikorwa cyo gukoraimiyoboro itandatu ya peptide synthesizer:

1. Tegura ibikoresho bibisi: hitamo aminide acide ikwiye, amatsinda arinda hamwe na reagent. Menya neza ko reagent zose hamwe na solve byumye kugirango wirinde hydrolysis.

2. Shira resin: Shyiramo aside amine acide mumurongo wa reaction ya synthesizer. Ibisigarira birashobora gukwirakwizwa muburyo butandatu kugirango habeho synthesis hamwe nubuziranenge bwa buri munyururu wa peptide.

3. Guhuza aside Amino: Kuvanga aside amine yifuzwa hamwe na reagent ikwiye hanyuma ukabishyira kumurongo wa reaction. Guhuza reaction mubisanzwe bifata igihe kugirango umenye neza ko aside amine ihambiriye rwose.

4. Kurandura Amatsinda Kurinda: Nyuma yo guhuza aside amine yose irangiye, amatsinda arinda agomba gukurwaho kugirango ashyire ahagaragara amatsinda amine yitegura icyiciro gikurikira cyo guhuza.

5. Isuku no de-activation: Nyuma yo kwangirika, ibisigazwa bigomba gusukurwa neza kandi amatsinda asigara akeneye gukora kugirango abuze kwivanga mubyakurikiyeho.

6. Inzinguzingo zikurikirana: Subiramo intambwe zavuzwe haruguru kugeza igihe peptide igenewe. Buri cyiciro gikeneye kwemeza guhuza aside amine no gukuraho burundu amatsinda arinda.

II. Ingingo z'ikoranabuhanga:

1. Guhitamo abatwara ibyiciro bikomeye: Guhitamo icyuma gikwiye (urugero, resin) ningirakamaro kuri synthesis ya peptide. Ubwoko na kamere ya resin bizagira ingaruka kumuvuduko no gukora neza.

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3. Guhitamo neza itsinda ririnda no kugenzura ibisabwa kugirango ryangirika ni urufunguzo rwo gutsinda kwa synthesis.

4. Kurandura imyanda: Imyanda n’imyanda idakorwa byakozwe mugihe cya synthesis bigomba gutabwa neza kugirango hagabanuke umwanda w’ibidukikije no kurinda umutekano wa laboratoire.

5. Kugenzura ubuziranenge: Mubikorwa byose bya synthesis, hasabwa ibizamini byo kugenzura ubuziranenge buri gihe kugira ngo buri ntambwe ya reaction ikorwe nkuko byari byateganijwe kandi ko peptide ikomatanya yujuje ibyateganijwe mbere nibisabwa kugira ngo bisukure.

Igikorwa cyaimiyoboro itandatu ya peptide synthesizerbisaba kugenzura neza imiti no gucunga neza inzira. Gusobanukirwa neza imikorere ya synthesizer hamwe nibikorwa bya tekinike nibyingenzi kugirango tunoze imikorere nubuziranenge bwa peptide.