Isomero

Imitwe

Amakuru y'ingenzi

Amakuru Agezweho ku Bushakashatsi: Peptide ya APC C-terminal APC11 itanga uburyo bushya bwo kuvura kanseri y'inkondo y'umura idakira ubudahangarwa bw'umubiri

Peptide nshya yavumbuwe mu bijyanye n'imitsi y'umutima: "Intwaro nshya" yo kugabanya umuvuduko w'amaraso no kurinda umutima—Alamandine- (1-5)

Molekuli za Peptide "Breaking Symmetry" Zituma ibikoresho bya Ferroelectric bifite ingufu nkeya kandi bigatera imbere mu mikurire y'imitsi

Guhuza imikorere y'ingirabuzimafatizo bigenzurwa bihura n'imikorere ya Ribosomal: Ivuka ry'urusobe rw'ingirabuzimafatizo rwa Bicyclic Peptide rw'Abagisekuru Gishya

Gukuraho ikibazo cyo guhindura Cysteine mu buryo bwo guhitamo: Ubutabire bwa Triazine-Pyridine butuma poroteyine ihuza neza

Iyo imiti irwanya imiyoboro y'amazi inaniwe, ese dufite gahunda yo kuyisubiza inyuma? Peptide zirwanya imikorobe ziragaragara nk'ibyiringiro bishya byo kurwanya "Superfungi"

Gutunganya Peptide Idafite Inyongeramusaruro Ikoresha Pentafluorophenyl Esters nk'Uburyo bwo Gutunganya Oligopeptide Ikoresheje Uburyo bwo Gutunganya Flow

Ivumburwa rya peptides zirwanya mikorobe mu mikorobe ku isi yose hamwe n'uburyo bwo kwiga imashini

Guhuza Tirzepatide hakoreshejwe hydrophobic tag-assisted liquid-Phase Synthesis

Iterambere Rigezweho! Gel Nshya yo mu bwoko bwa Hemostatic Gel itanga igisubizo gishya ku kuva amaraso mu duodenal diverticular

Uburyo bwo gukora bwa synthesizer ya peptide ifite imiyoboro itatu n'ibisobanuro birambuye ku mikorere

2024-06-21

Ihame ry'imikorere:

Igice cy'ingenzi cyasynthesizer ya peptide y'imiyoboro itatuni imiyoboro itatu yigenga yo guhuza, buri imwe ifite icyuma gisya, icyuma gitanga umwuka n'inkingi yo gukaraba. Izi ntambwe eshatu ni intambwe z'ingenzi za SPPS, kandi zemerera imiterere y'imikorere kugenzura no kunoza mu byiciro bitandukanye.

1. Imvange: Mu mvange ya buri muyoboro, aside amine zigomba kwitabwaho zivangwa n'ikintu gihuza ibintu kugira ngo habeho esters cyangwa analogues ziteguye kwitabwaho na resin matrix.

2. Reactor: Muri reactor, uruvange rwa esters active ruhuzwa na resin matrix kugira ngo habeho urunigi rushya rwa polypeptide. Mu kugenzura igihe cy'inyito, ubushyuhe na pH, reaction yo guhuza ishobora kunozwa kugira ngo igabanye amakosa n'ibikomoka kuri yo.

Inkingi yo gukaraba: Nyuma ya buri kongerwamo aside amine, inkingi yo gukaraba ikoreshwa mu gukuraho ibintu bitarakozweho n'ibikomoka kuri byo kugira ngo habeho urunigi rwa polypeptide rukozwe neza.

Twandikire

amakuru (1).png

II. Uburyo bwo gukora:

1. Gutangiza: Mbere yo gutangira, hagomba gutegurwa ibintu byose bihindura imikorere n'ibikoresho, harimo aside amine, igikoresho gihuza, matrix ya resin, isabune n'ibindi. Muri icyo gihe, synthesizer igomba gupimwa kugira ngo hamenyekane ko umuvuduko w'amazi, igitutu n'ibindi bipimo bya buri muyoboro ari ibisanzwe.

2. Gutegura Resin yo Gutangiza: Shyira resin yo gutangira muri buri muyoboro, akenshi resin yakoreshejwe, kugira ngo aside amine ya mbere ihuzwe.

3. Guhuza aside amine: aside amine ya mbere ivangwa n'ikintu gihuza hanyuma igashyirwa mu cyuma gihuza kugira ngo habeho ester ikora. Hanyuma, ester ikora ishyirwa mu cyuma gihuza na resin kugira ngo habeho peptide bond.

4. Gukaraba no Guhagarika: Resin yogezwa hakoreshejwe inkingi yo gukaraba kugira ngo ikureho aside amine zitarakora neza n'ikintu gihuza. Hanyuma, hakorwa intambwe yo guhagarika kugira ngo hakureho amatsinda yose asigaye ya ester akoreshwa.

5. Kongeramo aside amine zikurikirana: Subiramo intambwe zavuzwe haruguru, wongeramo aside amine zikurikiraho imwe imwe kugeza igihe urukurikirane rw'imiterere ya polipeptide wifuza rurangiye.

6. Guca no gutunganya uruhererekane rwa polypeptide: Nyuma yo guhuza aside amine yose, uruhererekane rwa polypeptide rugomba gutandukanywa na resin matrix hanyuma rugatunganywa. Ibi akenshi biba bisaba intambwe zo gusiga, gukaraba no kongera gutunganya.

7. Gutunganya terminal: Guhindura terminal bikorwa kuri peptide yabonetse, nko gukuraho amatsinda arinda terminal, n'ibindi bintu bikenewe mu kubungabunga chemical.

Itsindasynthesizer ya peptide y'imiyoboro itatuikora neza mu gukora peptide na poroteyine binyuze mu gukora poroteyine mu buryo bufatika. Ihame ryayo rishingiye ku buryo bwo gukora shimi bugenzurwa neza, mu gihe uburyo bwo gukora bukubiyemo intambwe nyinshi zigoye zisaba kugenzura neza imiterere y’imikorere.