Falanqee habka hawlgalka iyo qodobbada farsamada ee lix-channel peptide synthesizer

1. Habka hawlgalka eelix-channel peptide synthesizer:

1. Diyaari alaabta ceeriin: dooro xabagta amino acids ee ku haboon, kooxaha ilaalinta iyo reagents uumi. Hubi in dhammaan reagen-yaasha iyo dareerayaashu ay engegan yihiin si looga fogaado fal-celinta biyo-saarista.

2. Resin load: Ku shub resin amino acid galay tiirka falcelinta ee synthesizer. Resin-ka ayaa si siman loogu qaybin karaa lixda kanaal si loo hubiyo waxtarka isku-dhafka iyo tayada silsilad kasta oo peptide ah.

3. Isku-xidhka Amino Acids: Isku qas amino acids-ka la rabo iyo reagenyaal uumi ku habboon oo ku dar tiirka falcelinta. Dareen-celinta isku-xidhka ahi waxay caadi ahaan qaadataa wakhti si loo hubiyo in asiidhyada amino-yada ay si buuxda ugu xidhan yihiin resinka.

4. Ka saarida Kooxaha Difaaca: Ka dib marka la isku daro dhammaan asiidhyada amino-yada, kooxaha difaaca ayaa loo baahan yahay in meesha laga saaro si loo soo bandhigo kooxaha amino si loogu diyaar garoobo wareegga xiga ee isku xirka.

5. Nadiifinta iyo ka-hortagga shaqada: Ka-baxa ka dib, resin-ku wuxuu u baahan yahay in si fiican loo nadiifiyo iyo kooxaha falceliska ah ee hadhsan waxay u baahan yihiin in la damiyo si looga ilaaliyo inay farageliyaan falcelinta dambe.

6. wareegyada isdaba jooga: Ku celi tillaabooyinka kor ku xusan ilaa peptide bartilmaameedku uu ka samaysan yahay. Wareeg kastaa wuxuu u baahan yahay inuu hubiyo isku dhafka dhammaystiran ee asiidhyada amino iyo ka saarida si buuxda kooxaha difaaca.

II.Qodobada farsamada:

1. Xulashada side-adag: Xulashada qaade weji adag oo ku habboon (tusaale, resin) ayaa muhiim u ah isku-dhafka peptide. Nooca iyo dabeecadda resin-ku waxay saameyn doontaa xawaaraha iyo waxtarka isku-dhafka.

2. Falcelinta uumiga: Fal-celinta uumi waa tallaabo muhiim ah oo ka mid ah isku-dhafka peptide-ka, iyo reagents waxtarka leh ayaa loo baahan yahay in la doorto si loo hubiyo in isku xidhka asiidhyada amino-ku ay dhammaystiran tahay oo dib loo noqon karo.

3. Xeeladaha ilaalinta: Isku-dubaridka peptide, silsiladaha dhinaca ee asiidhyada amino-yada sida caadiga ah waxay u baahan yihiin in la ilaaliyo si looga hortago inay ka falceliyaan si aan loo baahnayn inta lagu jiro habka uumiga. Doorashada kooxda saxda ah ee ilaalinta iyo xakamaynta shuruudaha ilaalinteeda ayaa fure u ah guusha isku-dhafka.

4. Qashin qubka: Qashinka iyo reagen-yaasha aan la falgelin ee la dhaliyo inta lagu guda jiro habka isku-dhafka waxay u baahan yihiin in si sax ah loo tuuro si loo yareeyo wasakheynta deegaanka loona hubiyo badbaadada shaybaarka.

5. Xakamaynta tayada: Inta lagu jiro habka isku-dhafka, imtixaannada xakamaynta tayada joogtada ah ayaa loo baahan yahay si loo hubiyo in tallaabo kasta oo fal-celin ah loo fuliyo sidii loo qorsheeyay iyo in peptide-ka la isku daray uu buuxiyo qeexitaannada hore loo go'aamiyay iyo shuruudaha nadiifinta.

Hawlgalka eelix-channel peptide synthesizerwaxay u baahan tahay kontorool fal-celin kiimikaad oo wanaagsan iyo maarayn adag. Fahamka wanaagsan ee hababka hawlgalka synthesizer-ga iyo qodobbada farsamada ayaa lagama maarmaan u ah hagaajinta waxtarka iyo tayada isku-dhafka peptide.