Sekaseka ts'ebetso ea ts'ebetso le lintlha tsa tekheniki tsa synthesizer ea li-peptide tse tšeletseng

1. Ts'ebetso ea ts'ebetso easynthesizer ea likanale tse tšeletseng tsa peptide:

1. Lokisetsa lisebelisoa tse tala: khetha li-resin tse loketseng tsa amino acid, lihlopha tse sireletsang le li-reagents tsa condensation. Etsa bonnete ba hore li-reagents tsohle le li-solvents li omme ho qoba karabelo ea hydrolysis.

2. Roala resin: Kenya resin ea amino acid ka har'a kholomo ea karabelo ea synthesizer. Resin e ka ajoa ka mokhoa o lekanang liteisheneng tse tšeletseng ho netefatsa ts'ebetso e ntle ea motsoako le boleng ba ketane ka 'ngoe ea peptide.

3. Ho kopanya amino acid: Kopanya li-amino acid tse lakatsehang le li-reagents tse loketseng tsa condensation 'me u li kenye kholomong ea karabelo. Karabelo ea ho kopanya hangata e nka nako e itseng ho netefatsa hore li-amino acid li tlameletsoe ka ho feletseng ho resin.

4. Ho Tlosoa ha Lihlopha Tse Sireletsang: Ka mor'a hore ho kopanngoa ha li-amino acid tsohle ho phethoe, lihlopha tse sireletsang li lokela ho tlosoa e le hore li pepese lihlopha tsa amino bakeng sa ho itokisetsa potoloho e latelang ea ho kopanya.

5. Ho hloekisa le ho tima: Ka mor'a ho hloekisoa, resin e hloka ho hloekisoa hantle 'me lihlopha tse setseng tse sebetsang li lokela ho tlosoa ho li thibela ho kena-kenana le liphetoho tse latelang.

6. Lipotoloho tse latellanang: Pheta mehato e ka holimo ho fihlela peptide e reretsoeng e kopantsoe. Potoloho e 'ngoe le e' ngoe e hloka ho netefatsa ho kopanngoa ho feletseng ha li-amino acid le ho tlosoa ka ho feletseng ha lihlopha tse sireletsang.

II. Lintlha tsa theknoloji:

1. Khetho ea mochini o tsamaisang karolo e tiileng: Ho khethoa ha sejari se loketseng sa mokhahlelo o tiileng (mohlala, resin) ke sa bohlokoa bakeng sa motsoako oa peptide. Mofuta le mofuta oa resin li tla ama lebelo le katleho ea motsoako.

2. Karabelo ea condensation: Karabelo ea condensation ke mohato o ka sehloohong oa peptide synthesis, 'me li-reagents tse sebetsang hantle tsa condensation li hloka ho khethoa ho netefatsa hore tlamahano lipakeng tsa li-amino acid e felile ebile e ka khutlisoa.

3. Mekhoa ea ts'ireletso: Ka peptide synthesis, liketane tse ka thōko tsa amino acid hangata li hloka ho sireletsoa ho li thibela ho itšoara ka mokhoa o sa hlokahaleng nakong ea condensation. Ho khetha sehlopha se sireletsang se nepahetseng le ho laola maemo a tšireletso ea sona ke senotlolo sa katleho ea motsoako.

4. Tlhōlo ea litšila: Litšila le li-reagents tse sa sebetsanoeng tse hlahisoang nakong ea ts'ebetso ea motsoako li hloka ho lahloa ka nepo ho fokotsa tšilafalo ea tikoloho le ho netefatsa polokeho ea laboratori.

5. Taolo ea boleng: Ho pholletsa le ts'ebetso ea ho kopanya, liteko tsa kamehla tsa taolo ea boleng lia hlokahala ho netefatsa hore mohato o mong le o mong oa karabelo o etsoa joalo ka ha ho reriloe le hore peptide e kopaneng e kopana le lintlha tse reriloeng esale pele le litlhoko tsa bohloeki.

Ts'ebetso easynthesizer ea likanale tse tšeletseng tsa peptidee hloka taolo e ntle ea karabelo ea lik'hemik'hale le taolo e thata ea ts'ebetso. Kutloisiso e ntle ea lits'ebetso tsa ts'ebetso ea synthesizer le lintlha tsa tekheniki li bohlokoa ho ntlafatsa ts'ebetso le boleng ba peptide synthesis.