Hlaziya inqubo yokusebenza kanye namaphuzu obuchwepheshe we-peptide synthesizer eneziteshi eziyisithupha

1. Inqubo yokusebenza yei-peptide synthesizer eneziteshi eziyisithupha:

1. Lungiselela izinto ezingavuthiwe: khetha ama-amino acid resins afanelekile, amaqembu okuvikela kanye nama-reagents abushelelezi. Qinisekisa ukuthi wonke ama-reagents nezinyibilikisi zomile ukuze ugweme ukusabela kwe-hydrolysis.

2. Layisha inhlaka: Layisha i-amino acid resin kukholamu yokusabela ye-synthesizer. I-resin ingasatshalaliswa ngokulinganayo eziteshini eziyisithupha ukuze kuqinisekiswe ukusebenza kahle kwe-synthesis kanye nekhwalithi yeketango ngalinye le-peptide.

3. Ukuhlangana kwe-amino acid: Hlanganisa ama-amino acid owafunayo nama-reagents afanele wokujiya bese uwangeza kukholamu yokusabela. Ukusabela kokuhlangana kuvame ukuthatha isikhathi ukuze kuqinisekiswe ukuthi ama-amino acid aboshelwe ngokuphelele ereyini.

4. Ukususwa Kwamaqembu Avikelayo: Ngemva kokuqedwa kokuhlanganiswa kwawo wonke ama-amino acid, amaqembu avikelayo adinga ukususwa ukuze kuvezwe amaqembu amino acid ekulungiseleleni umzuliswano olandelayo wokuhlanganisa.

5. Ukuhlanza nokuvala ukusebenza: Ngemva kokuvinjelwa, inhlaka idinga ukuhlanzwa kahle futhi amaqembu asalayo asebenzayo adinga ukuvalwa ukuze avinjwe ukuthi angaphazamisi ukusabela okulandelayo.

6. Imijikelezo elandelanayo: Phinda izinyathelo ezingenhla kuze kube yilapho i-peptide eqondiwe isihlanganisiwe. Umjikelezo ngamunye udinga ukuqinisekisa ukuhlangana okuphelele kwama-amino acid kanye nokususwa okuphelele kwamaqembu okuzivikela.

II.Amaphuzu obuchwepheshe:

1. Ukukhethwa kwenkampani yenethiwekhi yesigaba esiqinile: Ukukhethwa kwenkampani yenethiwekhi yesigaba esiqinile (isb, inhlaka) kubalulekile ekuhlanganiseni kwe-peptide. Uhlobo nemvelo ye-resin izothinta isivinini nokusebenza kahle kokuhlanganiswa.

2. Ukusabela kokujiya: Ukusabela kwe-condensation kuyisinyathelo esibalulekile ekuhlanganiseni kwe-peptide, futhi ama-reagents e-condensation asebenzayo kudingeka akhethwe ukuze kuqinisekiswe ukuthi ukubopha phakathi kwama-amino acid kuphelele futhi kuyabuyiseleka emuva.

3. Amasu okuvikela: Ekuhlanganiseni kwe-peptide, amaketanga aseceleni ama-amino acid ngokuvamile adinga ukuvikelwa ukuze awavimbele ekuphenduleni ngokungadingekile phakathi nenqubo yokufingqa. Ukukhetha iqembu elivikelayo elilungile kanye nokulawula izimo zokuvinjelwa kwalo kuyisihluthulelo sempumelelo yokuhlanganiswa.

4. Ukulahlwa kwemfucuza: Imfucuza kanye nezinto ezisebenza kabusha ezingaphenduliwe ezikhiqizwa ngesikhathi sokuhlanganisa kudingeka zilahlwe kahle ukuze kuncishiswe ukungcoliswa kwemvelo nokuqinisekisa ukuphepha kwalabhorethri.

5. Ukulawulwa kwekhwalithi: Kuyo yonke inqubo yokuhlanganiswa, ukuhlolwa kokulawulwa kwekhwalithi okuvamile kuyadingeka ukuze kuqinisekiswe ukuthi isinyathelo ngasinye sokusabela senziwa njengoba kuhleliwe nokuthi i-peptide ehlanganisiwe ihlangabezana nokucaciswa okunqunywe kusengaphambili kanye nezidingo zobumsulwa.

Ukusebenza kwe-i-peptide synthesizer eneziteshi eziyisithuphakudinga ukulawulwa okuhle kokusabela kwamakhemikhali kanye nokuphathwa kwenqubo okuqinile. Ukuqonda kahle izinqubo zokusebenza ze-synthesizer kanye namaphuzu obuchwepheshe kubalulekile ukuze kuthuthukiswe ukusebenza kahle kanye nekhwalithi yokuhlanganiswa kwe-peptide.